賀建超; 石國昌
陜西省微生物研究所; 陜西省微生物研究所 西安; 西安

【中文摘要】 本文報道了使用蝸牛酶和纖維素酶從糙皮側(cè)耳和佛羅里達側(cè)耳雙核菌絲體中,成功地分離了高產(chǎn)量的原生質(zhì)體。菌絲體培養(yǎng)時間,混合酶液 pH 值,一定濃度的酶液用量,酶解溫度和時間與原生質(zhì)體形成相關。原生質(zhì)體的兩種再生型被觀察到,并計算了再生頻率,糙皮側(cè)耳為2.5%.佛羅里達側(cè)耳為2.3%。

【英文摘要】 The formation and regeneration of mycelial protoplasts from Pleu- rotus ostreatus and P.florida using snailase and cellulase were studied.The amount of release of protoplasts is related to mycelial age,buffer pH,temperature and dose of enzyme used.To achieve a better enzymolysis result,young mycelia, botained from the edge of colonies which were incubated on solid plates at 25℃ for 4 days,were used in this study.5ml 1% of snailase and cellulase was added to one gram mycelia (wet weight) in pH5.8 buffer for enzymolysis at 35℃ for 4.5 hours.The regeneration frequeucy of protoplasts of both strains were observed by using RM medium,2.5% for Pleurotus ostreatus and 2.3% for P.florida. Two regeneration types were obtained in LRM medium.For the first type,proto- plasts directly produced one or more germ tubes; but for the second,protoplasts produced bud-like structures at beginning and then develo ped into germ tubes.

【中文關鍵詞】 原生質(zhì)體; 再生型; 糙皮側(cè)耳; 佛羅里達側(cè)耳; 雙核菌絲體
【英文關鍵詞】 protoplasts; regeneration type; Pleurotus ostreatus; P.florida; two nuclear mycelia

【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,1989年05期 【DOI】CNKI:SUN:ZSYJ.0.1989-05-006