【作者】 田雪蓮;陸偉東;丁偉;卯霞;田雪珊;張軍

【Author】 TIAN Xue-lian1,LU Wei-dong1,DING Wei1,MAO Xia1,TIAN Xue-shan2,ZHANG Jun1(1.Yun-gui Plateau Institute of Biodiversity,Qujing Normal University,Qujing Yunnan 655011;2.Qujing Disease Control Center,Qujing Yunnan 655000)

【機構】 曲靖師范學院云貴高原生物多樣性研究所;曲靖市疾控中心

【摘要】 通過對Pm0217F菌株進行不同時間梯度的紫外誘變,得到5株不同的誘變菌株。通過對5個誘變株的菌落特征、生長速率、大孢子千分率、分生孢子不同聚集方式及蟲草菌素含量等生物學特性比較,結果顯示,誘變最適時間為60s,且大孢子比例高、擬青霉型產孢結構比例大的菌株生物學特性較優。

【Abstract】 Ultraviolet radiation to mutate Pm0217F were adopted,and five different strains were obtained at different times of UV mutagenesis.The colony morphology,growth rate,megaspore's permillage,conidiophore of different aggregation methods and content of cordycepin were compared.The results showed that the best time was 60 s,and the strain that had high proportion of megaspore and high proportion of Paecilomyces-type showed the best biological characters.

【關鍵詞】 蛹蟲草;分生孢子;蟲草菌素;擬青霉型

【Key words】 Cordyceps militaris;Conidiospore;Cordycepin;Paecilomyces-type

【基金】 曲靖師范學院科技創新團隊基金資助項目

【文獻出處】 中國食用菌, Edible Fungi of China, 編輯部郵箱 , 2010年05期

【DOI】CNKI:SUN:ZSYJ.0.2010-05-006