雙孢蘑菇性親和性相關分子標記的初步篩選
Primary Screening of Molecular Markers Related to Sexual Compatibility in Agaricus bisporus
詹才新 凌霞芬 楊家林
摘 要：以傳統的形態,生理生化分析和最新的DCS-PDMA性親和性測定方法為基礎,結合群體分離分析和RAPD技術對來源于同一雙孢蘑菇異核體菌株的12個不育同核原生質體個體進行分析,篩選與性親和性相關的分子標記.研究結果表明,供試的12個不育同核原生質體個體被分成兩大類性親和性類型,其中一類(A+)包括不育同核原生質體個體B、 C、 D、 E、 F、 G、 H、 I、 J、 L,另一類(A-)則僅僅包括不育同核原生質體個體K和M,同時篩選到一個與性親和性相關的分子標記OPA161500.從而為間接地利用雙孢蘑菇本身特有的交配型作標記來指導雜交育種工作和進一步將其性親和性基因定位分離克隆奠定了堅實的基礎.
關鍵詞：雙孢蘑菇; DCS-PDMA方法; 群體分離分析; 性親和性相關分子標記; 雜交育種
分類號：S464.110.36 文獻標識碼：A
文章編號：1005-9873(2002)04-01-08
基金項目：國家自然科學基金項目(95-01-04)
作者簡介：第一作者現在地址: Box 109, Laboratory of Developmental Neurobiology, the Rockefeller University,1230 York Avenue, New York, NY 10021, USA.楊家林，本文通訊作者
作者單位：詹才新（上海市農業科學院食用菌研究所;農業部食用菌遺傳育種重點開放實驗室,上海,201106）
凌霞芬（上海市農業科學院食用菌研究所）
楊家林（鄂州市衛生專科學校,鄂州,436000）
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