【作者】 李冠喜; 華國棟; 王多明; 魏良志;
【Author】 LI Guanxi1,2,HUA Guodong2,WANG Duoming2,WEI Liangzhi3 1Lianyungang City Academy of Agricultural Sciences,Lianyungang,Jiangsu 222006,China; 2College of Forest Resources and Environment,Nanjing Forestry University,Nanjing,Jiangsu 210037,China; 3Xinba Town Agricultural Technology Generalization Center of Haizhou District,Lianyungang,Jiangsu 222001,China
【機構】 連云港市農業科學院; 南京林業大學森林資源與環境學院; 連云港市海州區新壩鎮農業技術推廣中心;
【摘要】 采用同核體單孢雜交育種技術,以低溫高產的雙1菌株和高溫低產的雙5菌株為親本,選育雙孢蘑菇優良新品種。通過擔孢子分離和同核體的鑒別,獲得同核體菌株共20個,同核體間配對雜交組合99個,獲得10個雜交異核體菌株;經過菇房、出菇地棚栽培和出菇大棚生產性試驗,篩選出高產優質雜交菌株Z1。
【Abstract】 Monokaryotic single-spore isolates obtained from the high yield Shuang 1 strain and the low yield,high temperature Shuang 5 strain of Agaricus bisporus were used for breeding new high yielding,elevated temperature tolerant hybrids.Ninety-nine pairings were obtained from 20 monokaryotic spore isolates(11 from Shuang 1 and nine from Shuang 5,identified on the basis of isozyme profiles),and ten dikaryotic hybrids were obtained.After three cultivation cycles(carried out on a laboratory-scale,in 3 m2...
【關鍵詞】 雙孢蘑菇; 單孢雜交育種; 雜交;
【Key words】 Agaricus bisporus; cross-breeding using single spore isolates; hybridization;
【基金】 江蘇省農業三項工程項目[編號:sx(2009)133];連云港市農業科技成果示范計劃項目(編號:SF0902)的部分研究內容
【分類號】S646.11