【作者】王澄澈 梁枝榮

【機構】河南省洛陽農業高等專科學校；中國科學院北京農村經濟技術開發部；

【摘要】The protoplasts of auxotrophic mutant monokaryon of Pleurotus. sajor-caju was used as recipient parenL the protoplasts of wild dikareon of Lentinus edodes were heat-inactivated and used as nuclear donor parent. The fusants of two parents showed some their individual characteristics and physiological difference between themselfs. Thrugh protoplast re-isolation and reversion of one randomly selective fusant, two parents had been recovered and the donor parent achieved some new physiological characteristics such as faster mycelial growth and earier fruiting.This experiment result showed that the idea and the method of polarized protoplast fusion used in this study may be used as routine procedure for strain improvement in adible fungi.    

【關鍵詞】鳳尾菇; 香菇; 原生質體; 非對稱融合;