【作者】鄭維發 項小燕 陳才法 王堯 趙艷霞 蔣繼宏 儲成才
【機構】Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province Xuzhou Normal UniversitySchool of Biological Sciences Anqing Normal CollegeKey Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province Xuzhou Normal UniversityInstitute of Genetics and Developmental Biology Chinese Academy of SciencesXuzhou 221116 ChinaAnqing 26400 ChinaXuzhou 221116 ChinaBeijing 100101 China
【摘要】Lanosterol and ergosterol are the active principles with potential pharmacological activities in Inonotus obliquus. However, the two sterols are less accumulated in cultured mycelia of the fungus. In this study, different carbon and nitrogen sources and pH levels together with three metal ions were assayed for their effects on accumulation of the two sterols in the fungus. Among the tested media the growth medium consisting of glucose (1.5%), rice powder (0.5%), yeast extract (0.4%), wheat bran (0.1%), KH2PO4 (0.01%) and MgSO4·7H2O (0.05%) with pH level at 6.5 yielded a maximum production of the two sterols, which can further be increased following the treatment of Ag+, Cu2+ and Ca2+. Supplementing Ag+ at concentrations of 0.28 and 0.35μmol partially inhibited ergosterol biosynthesis, leading to an enhanced accumulation of lanosterol, the presence of intermediates of ergosterol biosynthetic pathway and a reduced accumulation of ergosterol in cultured mycelia of I.
【基金】：Natural Science Foundation of the Education Department of Jiangsu Province (No. 05KJA36012)； Natural Science Foundation of Jiangsu Province (BK2006034)；
【機構】Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province Xuzhou Normal UniversitySchool of Biological Sciences Anqing Normal CollegeKey Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province Xuzhou Normal UniversityInstitute of Genetics and Developmental Biology Chinese Academy of SciencesXuzhou 221116 ChinaAnqing 26400 ChinaXuzhou 221116 ChinaBeijing 100101 China
【摘要】Lanosterol and ergosterol are the active principles with potential pharmacological activities in Inonotus obliquus. However, the two sterols are less accumulated in cultured mycelia of the fungus. In this study, different carbon and nitrogen sources and pH levels together with three metal ions were assayed for their effects on accumulation of the two sterols in the fungus. Among the tested media the growth medium consisting of glucose (1.5%), rice powder (0.5%), yeast extract (0.4%), wheat bran (0.1%), KH2PO4 (0.01%) and MgSO4·7H2O (0.05%) with pH level at 6.5 yielded a maximum production of the two sterols, which can further be increased following the treatment of Ag+, Cu2+ and Ca2+. Supplementing Ag+ at concentrations of 0.28 and 0.35μmol partially inhibited ergosterol biosynthesis, leading to an enhanced accumulation of lanosterol, the presence of intermediates of ergosterol biosynthetic pathway and a reduced accumulation of ergosterol in cultured mycelia of I.
【基金】：Natural Science Foundation of the Education Department of Jiangsu Province (No. 05KJA36012)； Natural Science Foundation of Jiangsu Province (BK2006034)；