尚德靜; 王孝敏; 許永廷; 田星; 安悅
遼寧師范大學生命科學學院; 遼寧師范大學生命科學學院; 遼寧師范大學生命科學學院; 遼寧師范大學生命科學學院; 遼寧師范大學生命科學學院 大連116029 ; 大連116029 ; 大連116029 ; 大連116029 ; 大連116029
【中文摘要】 靈芝 (Ganodermalucidum)加硒深層培養后的菌絲 (含Se 16 0 0 μg/ g)分別經水及堿水提取、醇析、透析、Sevage法脫蛋白、DEAE cellulose柱層析純化 ,得SeGLP 1。經SephadexG 10 0、聚丙烯酰胺凝膠電泳和紫外光譜分析鑒定 ,SeGLP 1為均一級分。GC與TLC分析表明 ,SeGLP 1含Gal、Glu、Xyl、Rha、Man ,其單糖摩爾比為Gal∶Glu∶Xyl∶Rha∶Man =0 .2 3∶1.0 0∶0 .72∶ 0 .2 5∶ 0 .0 9。SeGLP 1硒含量 16 42 μg/g。SeGLP 1中Se活性中心的結構可能為O =Se =O ,即Se取代了靈芝多糖GLP 1中 -OCH3結構上的CH3,從而形成了硒氧雙鍵結構
【英文摘要】 The polysaccharide containing selenium in Ganoderma lucidum (SeGLP 1) was isolated through water, lye and alcohol extraction ,dialysis, deproteining with Sevage method and purifying by chromatography of DEAE cellulose column. And SeGLP 1 was identified to be a homogeneous fraction with Sephadex G 100 and polyacrylamide gel electrophoresis and UV spectrum analysis, while Gal, Glu, Xyl, Rha, Man were determined to be components of SeGLP 1 by GC and TLC. The quantitative analysis of SeGLP 1 showed that the molar ratio of Gal∶Glu∶Xyl∶Rha∶Man=0.23∶1.00∶0.72∶0.25∶0.09, and that SeGLP 1 contained 1642μg/g selenium. And the results from IR, 77 Se NMR, 13 C NMR and Laser Raman spectra showed that the structure of activity center of selenium of SeGLP 1 might be O=Se=O,and CH 3 in -OCH 3 group of GLP 1 was substituted by selenium forming this structure.
【中文關鍵詞】 靈芝; 硒多糖; 分離; 純化; 硒活性中心
【英文關鍵詞】 Ganoderma lucidum; Polysaccharide containing selenium (SeGLP); Isolation; Purification; Selenium activity center
【基金】遼寧省教委重點科研項目
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2002年01期 【DOI】CNKI:SUN:SYJB.0.2002-01-006