高躍; 袁萍; 茅仁剛; 陳浙江; 田翠
上海師范大學生命與環境學院; 上海師范大學生命與環境學院; 上海師范大學生命與環境學院; 上海師范大學生命與環境學院; 上海師范大學生命與環境學院 上海200234; 上海200234; 上海200234; 上海200234; 上海200234

【中文摘要】 研究了不同酶種類、酶濃度、酶解溫度和時間、滲透壓穩定劑、pH值及菌齡等因素對云芝原生質體制備以及不同再生培養基對云芝原生質體再生的影響。結果表明,菌齡3d的云芝菌絲體用3%溶壁酶,以pH5.5,0.4mol/L的KCl為穩定劑,30℃酶解5h獲得的原生質體產量最高;用RM1再生培養基培養,原生質體再生率最高。

【英文摘要】 We have examined the effects of selected parameters(nature and concentration of lytic enzyme,exposure time,temperature,nature,concentration and pH of osmotic stabilizer,mycelial age) on Coriolus versicolor protoplast production.Highest protoplast yields were obtained from 3-day-old mycelium after treatment with 3%(w/v) lywallzyme for 5 h at 30 ℃ using 0.4 mol/L KCl(pH 5.5) as osmotic stabilizer.Of six media tested,highest mycelium regeneration rates were obtained on RM1 medium consisting of 20%(w/v) potato,1%(w/v) yeast,0.3%(w/v) KH2PO4,0.15%(w/v) MgSO4,0.003%(w/v) Vitamin B1,0.4 mol/L KCl and 3%(w/v) agar.

【中文關鍵詞】 云芝; 原生質體制備; 原生質體再生
【英文關鍵詞】 Coriolus versicolor; protoplast preparation; protoplast regeneration

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2008年01期 【DOI】CNKI:SUN:SYJB.0.2008-01-016