BAO Dapeng; ZHANG Meiyan; LI Su; CHEN MingjieShanghai Research Center for Edible Fungi Biotechnology and Engineering; Key Laboratory of Agricultural Genetics and Breeding of Shanghai; Institute of Edible Fungi; Shanghai Academy of Agricultural Sciences; Shanghai 201106; Chin

【中文摘要】 A 508 bp fragment of a putative pheromone receptor gene from Lentinula edodes was amplified by PCR using a pair of degenerate primers designed according to conserved sequences of pheromone receptor genes reported in other Basidiomycetes.The fragment encodes 117 amino acids and contains three introns,56 bp,51 bp and 50 bp in size.Alignment analysis revealed that the deduced amino acid sequence of the L.edodes fragment exhibited 65% identity and 82% similarity with the corresponding segments of ph...更多eromone receptors reported in Coprinus cinereus and Schizophyllum commune.  還原

【英文摘要】 A 508 bp fragment of a putative pheromone receptor gene from Lentinula edodes was amplified by PCR using a pair of degenerate primers designed according to conserved sequences of pheromone receptor genes reported in other Basidiomycetes.The fragment encodes 117 amino acids and contains three introns,56 bp,51 bp and 50 bp in size.Alignment analysis revealed that the deduced amino acid sequence of the L.edodes fragment exhibited 65% identity and 82% similarity with the corresponding segments of pheromone receptors reported in Coprinus cinereus and Schizophyllum commune.

【英文關鍵詞】 Lentinula edodes; pheromone receptor; degenerate PCR
【基金】the Foundation of Shanghai Agaricultural Committee(No.2003-4-5);; Foundation of Shanghai Academy of Agricultural sciences(2007-24 and 2007-07)

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2008年02期 【DOI】CNKI:SUN:SYJB.0.2008-02-002