【作者】延光海; 李良昌; 沈光海; 李光昭
【Author】YAN Guanghai1; LI Liangchang1; SHEN Guanghai2; LI Guangzhao11Department of Anatomy; Yanbian University School of Basic Medical Sciences; Yanji; Jilin 133000; China; 2 College of Pharmacy; Yanbian University; Yanji; Jilin 133000; China
【作者機構】延邊大學醫學部解剖學教研部; 延邊大學醫學部藥學院
【摘要】 通過IgE誘導大鼠被動皮膚過敏反應(PCA)實驗、細胞毒性試驗、IgE誘導肥大細胞組胺釋放、鈣攝入量實驗、Western blot試驗,來研究樺褐孔菌(Inonotus oblipuus)乙醇提取物EEIO的抗過敏作用。試驗發現,EEIO明顯抑制了大鼠皮膚伊文思藍滲出,對IgE誘導肥大細胞的細胞內鈣攝入、組胺以及腫瘤壞死因子-α、白細胞介素6的釋放有劑量依賴抑制作用。表明,樺褐孔菌乙醇提取物具有一定的抗過敏作用。
【Abstract】 We have studied the anti-allergic effects of an orally-administered ethanolic extract of Inonotus obliquus(EEIO) by evaluating anti-DNP IgE-mediated passive cutaneous anaphylaxis(PCA) in rats,EEIO cytotoxicity,histamine release,calcium uptake,and TNF-α and IL-6 secretion by IgE-induced rat peritoneal mast cells(RPMCs) incubated with EEIO.EEIO(1 100 μg/mL) had no adverse effect on RPMC viability under the test conditions,and significantly inhibited Evans blue leakage in antigen-challenged rats sensitized by anti-DNP IgE.EEIO also suppressed antigen-stimulated calcium uptake and histamine release in RPMCs,and Western blot analysis revealed that EEIO inhibited the antigen-stimulated expression of TNF-α and IL-6 in RPMCs in a dose-dependent manner.Our data indicate that EEIO exhibits anti-allergic effects.
【關鍵詞】 樺褐孔菌; 肥大細胞; IgE; 組胺; 鈣; 腫瘤壞死因子-α; 白細胞介素6
【Key words】 Inonotus obliquus; mast cell; IgE; histamine; calcium; TNF-α; IL-6
【基金】吉林省教育廳項目(編號:吉教科合字[2009]第28號)的部分研究內容~~
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2010年02期
【DOI】CNKI:SUN:SYJB.0.2010-02-014
【分類號】R285.5