【作者】延光海; 李良昌; 沈光海; 李光昭

【Author】YAN Guanghai1; LI Liangchang1; SHEN Guanghai2; LI Guangzhao11Department of Anatomy; Yanbian University School of Basic Medical Sciences; Yanji; Jilin 133000; China; 2 College of Pharmacy; Yanbian University; Yanji; Jilin 133000; China

【作者機(jī)構(gòu)】延邊大學(xué)醫(yī)學(xué)部解剖學(xué)教研部; 延邊大學(xué)醫(yī)學(xué)部藥學(xué)院

【摘要】 通過IgE誘導(dǎo)大鼠被動(dòng)皮膚過敏反應(yīng)(PCA)實(shí)驗(yàn)、細(xì)胞毒性試驗(yàn)、IgE誘導(dǎo)肥大細(xì)胞組胺釋放、鈣攝入量實(shí)驗(yàn)、Western blot試驗(yàn),來研究樺褐孔菌(Inonotus oblipuus)乙醇提取物EEIO的抗過敏作用。試驗(yàn)發(fā)現(xiàn),EEIO明顯抑制了大鼠皮膚伊文思藍(lán)滲出,對(duì)IgE誘導(dǎo)肥大細(xì)胞的細(xì)胞內(nèi)鈣攝入、組胺以及腫瘤壞死因子-α、白細(xì)胞介素6的釋放有劑量依賴抑制作用。表明,樺褐孔菌乙醇提取物具有一定的抗過敏作用。

【Abstract】 We have studied the anti-allergic effects of an orally-administered ethanolic extract of Inonotus obliquus(EEIO) by evaluating anti-DNP IgE-mediated passive cutaneous anaphylaxis(PCA) in rats,EEIO cytotoxicity,histamine release,calcium uptake,and TNF-α and IL-6 secretion by IgE-induced rat peritoneal mast cells(RPMCs) incubated with EEIO.EEIO(1 100 μg/mL) had no adverse effect on RPMC viability under the test conditions,and significantly inhibited Evans blue leakage in antigen-challenged rats sensitized by anti-DNP IgE.EEIO also suppressed antigen-stimulated calcium uptake and histamine release in RPMCs,and Western blot analysis revealed that EEIO inhibited the antigen-stimulated expression of TNF-α and IL-6 in RPMCs in a dose-dependent manner.Our data indicate that EEIO exhibits anti-allergic effects.

【關(guān)鍵詞】 樺褐孔菌; 肥大細(xì)胞; IgE; 組胺; 鈣; 腫瘤壞死因子-α; 白細(xì)胞介素6

【Key words】 Inonotus obliquus; mast cell; IgE; histamine; calcium; TNF-α; IL-6

【基金】吉林省教育廳項(xiàng)目(編號(hào):吉教科合字[2009]第28號(hào))的部分研究內(nèi)容~~

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2010年02期

【DOI】CNKI:SUN:SYJB.0.2010-02-014
 
【分類號(hào)】R285.5