【作者】周艷玲; 陳文穎; 趙雯; 趙敏
【Author】ZHOU Yan-ling1; CHEN Wen-ying2; ZHAO Wen1; ZHAO Min1 (1.Department of Life Science; Northeast Forestry University; Harbin Heilongjiang 150040; 2.Heilongjiang Radio&Television University; Harbin Heilongjiang 150040)
【作者機構】東北林業大學生命科學學院; 黑龍江廣播電視大學
【摘要】 研究北蟲草超氧化物歧化酶(SOD)的分離純化和部分酶學特性。結果表明,從北蟲草中提取SOD的最佳工藝條件為原料與PBS(pH7.73)配比為1∶3(m∶v),提取時間為3h,純化倍數可達到粗酶液的4.09倍。提純后的SOD在690nm左右出現了特征吸收峰,最適溫度38℃,最適pH值為7.8,大部分糖對SOD的活性沒有顯著影響,有機酸都有降低酶活性的作用,過氧化氫對該酶有抑制作用,北蟲草SOD是以銅和鋅離子為輔基,為北蟲草的進一步開發和利用提供了理論依據。
【Abstract】 The properties of superoxide dismutase (SOD) from Cordyceps militaris were studied. The optimal isolating conditions were that the ratio of crude extract and PBS was 1∶3 (m∶v) , the pH value of PBS was 7.73, and the extraction time was 3 h. The purification multiple was 4.09 by column chromatography. The purified SOD showed characteristic peak at 690 nm. The optimum temperature and pH were 38℃ and 7.8, respectively. Lactose, sucrose, glucose and maltose had no impact on the activity of SOD. The activity of SOD was inhibited by organic acid and H2O2. SOD of C. militaris was Cu, Zn-SOD. SOD from C. militaris were isolated and purified successfully, and its partial enzymatic properties were studied, which provided important information for future applications and research.
【關鍵詞】 北蟲草; SOD; 純化; 酶學特性
【Key words】 Cordyceps militaris; Superoxide dismutase (SOD) ; Purification; Enzymatic properties
【基金】東北林業大學青年科研基金項目(09054)
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2010年04期
【DOI】CNKI:SUN:ZSYJ.0.2010-04-017
【分類號】S567.35