Mating Type Factor A and Mating Pheromone Production of Tremella fuciformis

Abstract：Eight strains of Tremella fuciformis were studied. Intrastrain and interstrain cross between single spore isolated from the eight strains indicated that the mating type factor A were biallelic.The effects of culture condition on mating pheromone production by T. fuciformis spores was studied. Single factor and orthogonal tests indicated the optimal culture condition for mating pheromone production was sucrose 20 g/L, (NH4)2SO4 12 mmol/L, MgSO4.*7H2O 0.25 g/L, KH2PO4*3H2O 0.5 g/L,VB1 0.2 mg/L,ZnSO4*7H2O 2 mg/L,CaCl2.*2H2O 0.5 g/L,(NH4)6Mo7O24*4H2O 0.02 mg/L,23 ℃,pH 6.5, 140 r/min. In the optimal culture condition, the highest activity was detected at the culture time of 4 days.The mating pheromone was completely inactivated by the treatment with pepsin, suggesting that the mating pheromone produced by H32 is a kind of peptide.

Keywords：Tremella fuciformis; mating type factor; mating pheromone

銀耳交配型因子及交配信息素的初步研究

盛桂華　 陳立國　 馬愛民　

摘　要：以8個銀耳菌株為材料,研究交配型因子A在不同菌株中的分布,菌株內及菌株間單孢分離物配對試驗結果表明,此8個菌株中只有兩個不同的A因子,與前人報道的銀耳是限制型雙因子交配系統的報道是一致的.研究了培養條件對銀耳孢子產交配信息素的影響, 結果表明銀耳孢子產交配信息素的較佳培養基為: 蔗糖 20 g/L ,(NH4)2SO4 1.584 g/L(含N 24 mmol/L), MgSO4*7H2O 0.25 g/L , KH2PO4*3H2 O 0.5 g/L , VB1 0.2 mg/L , ZnSO4*7H2O 2 mg/L , CaCl2*2H2O 0.5 g/L,鉬酸銨0.02 mg/L.最佳發酵條件為培養基pH 6.5,23 ℃,140 r/min,在優化培養基和培養條件下,培養4 d發酵液中交配信息素的活性最高,可達到12 U/mL,是在基礎培養基中活力的5～6倍.銀耳交配信息素經胃蛋白酶處理后,活性完全喪失,由此推測,銀耳交配信息素是一種多肽類物質.

關鍵詞：銀耳; 交配型因子; 交配信息素

CLC Number：S646.601

Foundation Item：Supported by a grant from The Center for Microbial Resources, The Institute of Microbiology of Chinese Academy of Sciences (IMCAS)

Author Resume：Sheng Guihua, born in 1976, master of College of Plant Science and Technology,Huazhong Agricultural University, Wuhan 430070, China

Author Unit：盛桂華（華中農業大學植物科學與技術學院,武漢,430070）　

　　　　　陳立國（華中農業大學植物科學與技術學院,武漢,430070）　

　　　　　馬愛民（華中農業大學植物科學與技術學院,武漢,430070）　

References：

［1］Bandoni R J.The Tremellales and Auriculariales: an alternative classification. Trans Mycol Soc Japan, 1984, 25:489～530

［2］Fox R D, Wang G J.Homothallism and heterothallism in Tremella fuciformis .Canada J Bot, 1990, 68:107～111

［3］Wang G J, Wells K. Modified bifactorial incompatibility in Tremella mesenterica .Trans Brit Mycol Soc, 1985, 84: 95～109

［4］Cheng M.Study on the conditions of basidiospore germination and determination of polarity of Tremella fuciformis:[Master Thesis].Wuhan: Huazhong Agricultural University,1999. 1～6

［5］Sheng G H. Determination of mating type factor A and primary studies on mating pheromone of Tremella fuciformis:[Master Thesis].Wuhan: Huazhong Agricultural University,2002.1～6

［6］Tilman S. Pheromone trigger filamentous growth in Ustilago maydis. The EMBO Journal ,1994, 13: 1620～1627

［7］Tsuchiya E, Fukui S. Biological activity of sex hormone produced by Tremella mesenterica. Agri Biol Chem, 1978, 42: 1089～1091

華中農業大學學報

JOURNAL OF HUAZHONG AGRICULTURAL UNIVERSITY

2004 Vol.23 No.1