徐朝暉; 姜世明; 付培武
山東師范大學(xué)和物系; 山東省動(dòng)物抗性重點(diǎn)實(shí)驗(yàn)室

【中文摘要】 雙孢蘑菇子實(shí)體（Ａｇａｒｉｃｕｓｂｉｓｐｏｒｕｓ）經(jīng)熱水提取，乙醇沉淀，胰蛋白酶消化，Ｓｅｖａｇ法去除蛋白質(zhì)等處理，得多糖。經(jīng)紫外可見(jiàn)光分光光度計(jì)測(cè)定無(wú)紫外吸收峰，為較純多糖。并研究了其對(duì)人肝癌ＳＭＭＣ－７７２１細(xì)胞生長(zhǎng)曲線、有絲分裂指數(shù)及線粒體活性的影響，結(jié)果表明該多糖對(duì)體外培養(yǎng)的人肝癌ＳＭＭＣ－７７２１細(xì)胞增殖有一定抑制作用，抑癌實(shí)驗(yàn)證明該多糖對(duì)小鼠Ｓ－１８０實(shí)體瘤亦有一定抑制作用。

【英文摘要】 The polysacchride was isolated from the fruit bodies of Agaricus bisporus Sing. and verified by ultraviolet spectrum. The proliferation of human hepatoma SMMC-7721 cell line was significantly inhibited by the treatment of 50ug/mL the polysacchride. The conclusion was proved by determining the variation of the growth curve, mitosis index and mitochodria activity. The inhibitory activity of the polysacchride against implanted S-180 tumor in mice was also perfromed with the 51.9% inhibition.

【中文關(guān)鍵詞】 雙孢蘑菇子實(shí)體; 多糖; 抑瘤
【英文關(guān)鍵詞】 Agaricus bisporus Sing; polysacchride
【基金】山東省科委資助

【文獻(xiàn)出處】 中國(guó)食用菌,EDIBLE FUNGI OF CHINA,編輯部郵箱,1997年04期 【DOI】CNKI:SUN:ZSYJ.0.1997-04-001