羅信昌
華中農(nóng)業(yè)大學(xué)!湖北武漢430070

【中文摘要】 按照植物病理學(xué)原則 ,蘑菇分離物應(yīng)依據(jù)柯赫法則 (Koch’spostulate)回接。在一定條件下 ,人工誘發(fā)子實(shí)體得以鑒別。但是一些蘑菇種類 (如某些共生食用蘑菇 )難以人工誘發(fā)子實(shí)體 ,即使能形成子實(shí)體也費(fèi)時(shí)費(fèi)力。本文介紹DNA指紋鑒別技術(shù) ,即運(yùn)用RAPD -PCR技術(shù)制成分離物及對(duì)應(yīng)子實(shí)體指紋圖譜 ,分析它們間DNA同源性及遺傳相似度以鑒別分離物真?zhèn)?,探求快速 ;簡(jiǎn)便 ;有效的鑒別方法。

【英文摘要】 The isolate of mushrooms should be identified by back inoculation,artificial induce sporophore formation under adapted condition according to Koch's postulate. However,it is very difficult to form sporophore by artificial inducing in some species,i.g.ectomycorrhizal mushrooms.Even if sporophore formation,it would take a long time and lots of efforts.Isolate would be identified by application PAPD-PCR technique,to make DNA band,analysis DNA homogeneity,DNA similarity coefficient between isolate and sporophore,which is of speed,handy and availble for characterization isolate of mushroom.

【中文關(guān)鍵詞】 蘑菇; 分離物; RAPD-PCR; DAN圖譜
【英文關(guān)鍵詞】 Mushrooms; Isolate; RAPD-PCR; DNA band
【基金】國家自然科學(xué)基金!39770 0 2 5 ;; 高等學(xué)校博士學(xué)科點(diǎn)專項(xiàng)基金資助項(xiàng)目部分內(nèi)容

【文獻(xiàn)出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2001年03期 【DOI】CNKI:SUN:ZSYJ.0.2001-03-000