黃志立; 黃彥君; 張麗君; 劉冬; 梁世中
深圳職業(yè)技術(shù)學(xué)院化生學(xué)院應(yīng)用生物技術(shù)系; 華南理工大學(xué)生物工程系

【中文摘要】 通過(guò)設(shè)計(jì)L16(4)5正交實(shí)驗(yàn),確定了可提高云芝菌絲體中SOD活性的深層發(fā)酵培養(yǎng)基的最優(yōu)組分,實(shí)驗(yàn)結(jié)果表明,培養(yǎng)基各組分對(duì)菌絲體生物量的影響順序依次為黃豆粉>(NH4)2SO4>土豆>葡萄糖>麥麩,而對(duì)SOD總活力的影響順序?yàn)?NH4)2SO4>黃豆粉>麥麩>葡萄糖>土豆。綜合考慮試驗(yàn)結(jié)果及成本因素,確定云芝菌絲體產(chǎn)SOD的最適培養(yǎng)基為馬鈴薯160g·L-1、葡萄糖16g·L-1、麥麩10g·L-1、黃豆粉18g·L-1,不添加硫酸銨,pH自然。

【英文摘要】 In this paper, the effects of culture ingredients on the mycelia growth and SOD activity of Coriolus vericolor were observed using an orthogonal layout L16 (45). Results showed that the order of the effects of culture ingredients on the mycelial growth of Coriolus vericolor were soybean powder> (NH4)2SO4>potato>glucose>wheat bran, while the order of the effects of the components on SOD activity were (NH4)2SO4>wheat bran>soybean powder>glucose>potato. The optimal concentration of medium was as follows: 160 g·L-1 potato, 16 g·L-1 glucose, 10 g·L-1 wheat bran, 18 g·L-1 soybean powder and natural pH.

【中文關(guān)鍵詞】 云芝; 菌絲體; SOD; 深層發(fā)酵; 培養(yǎng)基; 優(yōu)化
【英文關(guān)鍵詞】 Coriolus vericolor; Mycelia; SOD; Optimization; Submerged culture; Medium ingredients
【基金】深圳職業(yè)技術(shù)學(xué)院重點(diǎn)科技開(kāi)發(fā)項(xiàng)目(01KJb016),本研究已授權(quán)國(guó)家發(fā)明專(zhuān)利,專(zhuān)利號(hào):ZL200410027125.X

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2009年04期 【DOI】CNKI:SUN:ZSYJ.0.2009-04-020