黃碧芳; 朱鴻萍; 李彩斌; 林文雄
福建農林大學生命科學學院

【中文摘要】 以溫度40℃處理為實驗組,10℃處理為對照組,采用雙向電泳技術,比較了虎奶菇菌絲體蛋白質的3種提取方法,其中TCA/丙酮法提取所得到的蛋白質樣品的雙向電泳圖譜效果最佳,為其它大型真菌蛋白質的提取提供了重要參考。并對TCA/丙酮法加以改進,獲得虎奶菇菌絲體胞外蛋白質樣品的制備方法,為應用差異蛋白質組學方法對虎奶菇菌絲體自溶進行研究奠定基礎。

【英文摘要】 In this study,the Pleurotus tuber-regium(Fr.) Sing mycelia processing 40℃was used as test group and those with 10℃treatment served as conrol group.Three methods of extracting mycelium proteins were compared.The results showed that the TCA/acetone method is optimal with regard to the electrophoretic effect of protein sample,which provided useful guidance for the protein prepartion of other macromycetes.The TCA/acetone method was adjusted and improved in this study to be suited for extracting the extracellular proteins of the Pleurotus tuber-regium(Fr.) Sing mycelium,providing the basis for investigating the mycelium autolysis of the Pleurotus tuber-regium(Fr.) Sing with differential proteomic method.

【中文關鍵詞】 虎奶菇; 蛋白質提取; 2-DE
【英文關鍵詞】 Pleurotus tuber-regium(Fr.) Sing; Protein extraction; 2-DE
【基金】福建農林大學校青年基金項目“虎奶菇菌絲自溶的差異蛋白組學分析”(06A10)

【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2010年01期 【DOI】CNKI:SUN:ZSYJ.0.2010-01-020