馬愛民 ; 賀冬梅 ; 凌霞芳 ; 潘迎捷
上海市農業科學院食用菌研究所 ; 農業部食用菌遺傳育種重點實驗室 上海 201106

【中文摘要】 為建立雙孢蘑菇同核原生質體雜交育種體系,本文對同核原生質體親合性反應及雜交異核體F_1代的農藝性狀和F_2代的分離與變異規律進行了研究。結果表明:雙孢蘑菇同核原生質體的雜交是一個較復雜的性親合與核遷移過程,其雜交率較低。在按氣生型×氣生型、氣生型×匍匐型、匍匐型×匍匐型組合的520個雜交配對中,共獲得113個雜交異核體,平均雜交率為22%。本研究同時建立了以菌落形態、菌絲生長速度、羧甲基纖維素酶相對活性以及酯酶同功酶電泳為手段的雜交異核體鑒定體系。

【英文摘要】 Compatible reaction of homokaryotic protoplasts, agronomic characters of heterokaryotic hybrid F1 generations, separation and variation of F2 generations were studied in this paper to establish a cross breeding system of homokaryotic protoplast in Agaricus bisporus. Results showed that cross between homokaryotic protoplast in Agaricus bisporus was a complicated procedure of sex compatibility and nuclear migration and its hybridization percentage was not high. 113 heterokaryotic hybrids were obtained from 520 combinations pairing with fluffy type x fluffy type, fluffy type x appressed type, appressed type x appressed type and average hybridization percentage was 22%. Meanwhile, identification system for heterokaryotic hybrid was also established by means of colonial morphology, mycelial growth rate, relative activity of Cx enzyme and esterase isozyme elecrophoresis.

【中文關鍵詞】 雙孢蘑菇; 同核原生質體; 雜交育種; 親合性反應; 雜交異核體; F_1代與F_2代; 農藝性狀; 分離與變異
【英文關鍵詞】 Agaricus bisporus; Homokaryotic protoplast; Cross breeding ; Compatible reaction; Heterokaryotic hybrid; F_1 and F_2 generation; Agronomic character; Separation and variation
【基金】上海市科委資助項目

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1995年04期 【DOI】CNKI:SUN:SYJB.0.1995-04-001