周洪英; 邊銀丙
湖北省農業科學院經濟作物研究所; 華中農業大學應用真菌研究所 武漢430070; 武漢430070

【中文摘要】 以蛹蟲草(Cordyceps militaris)4號和8號菌株作為親本,采用滅活原生質體融合技術獲得228個再生菌株。酯酶同工酶與ISSR-PCR方法相結合鑒定融合子,以蟲草素含量和子實體性狀為篩選指標,經過初步的遺傳穩定性測試,得到蟲草素含量顯著提高的5個穩定融合子——CM59、CM61、CM69、CM136和CM186 ,其蟲草素含量分別為8號菌株的1 .17 1 .73倍。實驗證明融合子菌絲中蟲草素含量普遍提高。篩選得到的5個融合子經進一步遺傳穩定性驗證后可用于以獲得蟲草素為目的的菌絲體發酵培養。

【英文摘要】 Protoplast fusion was carried out between protoplasts prepared from two parental strains of Cordyceps militaris (strains 4 and 8) after the protoplasts from both parental types had first been inactivated either by exposure to 60 ℃ or to UV irradiation. A total of 228 putative fusion products were obtained following protoplast regeneration and confirmed as true protoplast-fusion products by esterase isozyme analysis and ISSR-PCR methodology. Five regenerated fusion products, designated CM59, CM61, CM69, CM136 and CM186, were selected for increased and stable cordycepin production capacity. Increased cordycepin production ranged from between 1.17～1.73-fold higher compared with the higher cordycepin-yielding parental strain 8. While most of the fusion products exhibited increases in cordycepin content, the five selected isolates appear to have a high potential for producing cordycepin in fermentation systems subject to further testing of genetic stability.

【中文關鍵詞】 滅活原生質體; 原生質體融合; 鑒定; 蟲草素; 遺傳穩定性
【英文關鍵詞】 Inactivated protoplast; Protoplast fusion; Cordycepin; Genetic stability
【基金】華中農業大學各類人才科研啟動項目(No52204-05021)的部分研究內容

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2007年02期 【DOI】CNKI:SUN:SYJB.0.2007-02-016