崔月花; 楊艷彬; 章克昌
揚(yáng)州大學(xué)生物科學(xué)與技術(shù)學(xué)院; 內(nèi)蒙古赤峰市紅山區(qū)建設(shè)局; 江南大學(xué)教育部工業(yè)生物技術(shù)重點(diǎn)實(shí)驗室 江蘇揚(yáng)州225009; 內(nèi)蒙古赤峰024000; 江蘇無錫214036

【中文摘要】 研究了7種藥食兼用中藥薏苡仁(Coix lachryma-jobi)、苦蕎[Fagopyrum tataricum(L.) Gaertn]、桑葉(Morus albaL.)、銀杏葉(Ginkgo biloba L.)、苦竹葉(Pleioblastus amarusKeng f.)、生姜(Zingiber officinale Rosc.)和枸杞(Lycium chinense Mill.)對靈芝菌絲生長和靈芝發(fā)酵產(chǎn)物活性成分的影響。研究結(jié)果表明,薏苡仁對靈芝菌絲生長和靈芝發(fā)酵產(chǎn)物清除自由基有促進(jìn)作用。當(dāng)薏苡仁添加濃度為6.6 g/L時,靈芝菌絲體生物量為10.4 g/L±0.4 g/L,比不加中藥的對照高1.6 g/L±0.2 g/L;添加薏苡仁后,靈芝發(fā)酵產(chǎn)物對羥自由基(hydroxyl radical)、超氧陰離子(superoxide anion)和二苯代苦味酰肼(1-diphenyl-2-picryl-hydrazyl, DPPH )的清除率分別為64.5%±0.8%, 38.5%±0.4%和56.5%±0.5%,比不加中藥的對照高9.7%±0.4%、8.6%±0.2%和9.6...更多%±0.4%。高效液相色譜(high performance liquid chromatography,HPLC)分析表明,添加薏苡仁的靈芝發(fā)酵產(chǎn)物中可能出現(xiàn)了新的活性成分。  還原

【英文摘要】 The effect of seven herbs [Coix lachryma-jobi,Fagopyrum tataricum(L.) Gaertn,Morus alba L.,Ginkgo biloba L.,Pleioblastus amarus Keng f.,Zingiber officinale Rosc.and Lycium chinense Mill.] on Ganoderma lucidum biomass production in submerged culture,and on the levels of free-radical scavenging fungal fermentation products in the spent culture fluid,was determined.Mycelial biomass was significantly higher in cultures supplemented with C.lachryma-jobi(10.4±0.4 g/L),F.tataricum(10.5±0.5 g/L),L.chinense(10.7±0.6 g/L) and Z.officinale(10.5±0.6 g/L) compared with unsupplemented controls(8.8±0.6 g/L).Highest increases in scavenging activity towards hydroxyl,superoxide anion and 1-diphenyl-2-picrylhydrazyl(DPPH) radicals were recorded in cultures supplemented with C.lachryma-jobi(64.5%±0.8%,38.5%±0.4%,and 56.5%±0.5%,respectively,compared with control levels of 54.8%±0.6%、29.9%±0.3%和46.9%±0.4%,respectively.Increased scavenging activity towards hydroxyl and DPPH radicals was also recorded in cult...更多ures supplemented with Z.officinale and M.alba,respectively.An additional peak was detected by high performance liquid chromatography(HPLC) among the fermentation products in G.lucidum cultures supplemented with C.lachryma-jobi.  還原

【中文關(guān)鍵詞】 靈芝; 中藥; 生物量; 自由基清除活性; 高效液相色譜
【英文關(guān)鍵詞】 Ganoderma lucidum; edible herb; fungal biomass; free-radical scavenging activity; high performance liquid chromatography(HPLC)

【文獻(xiàn)出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,2008年01期 【DOI】CNKI:SUN:SYJB.0.2008-01-014