張美彥; 鮑大鵬; 陳明杰; 尚曉冬; 譚琦
農(nóng)業(yè)部應(yīng)用真菌資源與利用重點(diǎn)開(kāi)放實(shí)驗(yàn)室; 上海市食用菌工程技術(shù)研究中心; 上海市農(nóng)業(yè)遺傳育種重點(diǎn)實(shí)驗(yàn)室; 上海市農(nóng)業(yè)科學(xué)院食用菌研究所
【中文摘要】 根據(jù)香菇(Lentinula edodes)編碼線粒體中間肽酶le-mip基因的保守序列,通過(guò)基因組步行法對(duì)le-mip基因及其上游、下游區(qū)域進(jìn)行步行擴(kuò)增,擴(kuò)增產(chǎn)物經(jīng)SeqMan程序拼接后再BlastX搜索,并進(jìn)行同源性分析。所獲得的序列長(zhǎng)8880bp,其中有5個(gè)推定的基因,且與le-mip緊密連鎖的基因中沒(méi)有香菇的A交配型基因。
【英文摘要】 Based on a previously isolated conserved mip gene fragment,chromosome walking was used to amplify the mitochondrial intermediate peptidase gene (mip) in Lentinula edodes,together with the upstream and downstream genomic sequences. Amplified products were connected by SeqMan software,subjected to a BlastX search,and aligned. The total length of combined sequences was 8 880 bp,which together comprised four complete and one partial gene sequences. The A mating type gene in L. edodes is not linked to le-mip.
【中文關(guān)鍵詞】 香菇; 基因組步行; le-mip基因
【英文關(guān)鍵詞】 Lentinula edodes; chromosome walking; le-mip gene
【基金】上海市浦江人才計(jì)劃(編號(hào):08PJ14087);; 上海市農(nóng)業(yè)科學(xué)院資助項(xiàng)目(編號(hào):農(nóng)科發(fā)2007-07和農(nóng)科發(fā)2007-24)的部分研究?jī)?nèi)容~~
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2009年02期 【DOI】CNKI:SUN:SYJB.0.2009-02-005