【作者】周帥; 唐慶九; 楊焱; 賈薇; 劉艷芳; 唐傳紅; 馮娜; 劉方; 張勁松
【Author】ZHOU Shuai; TANG Qingjiu; YANG Yan; JIA Wei; LIU Yanfang; TANG Chuanhong; FENG Na; LIU Fang; ZHANG Jinsong National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization; Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Research Center of Natural Medicinal Resources; Institute of Edible Fungi; Shanghai Academy of Agricultural Sciences; Shanghai 201106; China
【作者機構】國家食用菌工程技術研究中心; 農業部應用真菌資源與利用重點開放實驗室; 上海市農業遺傳育種重點開放實驗室; 上海市農業科學院天然藥用資源研究與開發中心; 上海市農業科學院食用菌研究所
【摘要】 以藥用真菌姬松茸(Agaricus blazei)、雞腿菇(Coprinus comatus)、猴頭(Hericium erinaceus)、灰樹花(Grifola frondosa)和靈芝(Ganoderma lucidum)的水提多糖為材料,采用二喹林甲酸法、考馬斯亮藍法、福林-酚法、紫外分光光度法、離子色譜法和傳統凱氏定氮法對其中的蛋白含量進行測定和比較分析。結果表明,不同測定方法獲得的蛋白含量測定值有差異;筆者推薦使用離子色譜法進行精確蛋白含量測定,采用福林-酚法進行快速趨勢比較測定。
【Abstract】 Protein in crude polysaccharide material from five medicinal fungi (Agaricus blazei,Coprinus comatus,Hericium erinaceus,Grifola frondosa,Ganoderma lucidum),prepared by hot (100 ℃) aqueous extraction,was determined using six procedures:bicinchoninic acid. assay,Bradford,Lowry,Kjeldahl,UV spectrophotometry and high performance anion exchange chromatography (HAPEC-PAD). Different protein values were obtained using each procedure,and it is proposed that HAPEC-PAD and the Lowry method be adopted for the accurate and approximate determination of protein in fungal polysaccharide preparations,respectively.
【關鍵詞】 藥用真菌; 多糖; 蛋白含量; 福林-酚法; 離子色譜法
【Key words】 Medicinal fungi; polysaccharide; protein content; Lowry method; high performance anion exchange chromatography
【基金】上海市農業科學院青年基金項目的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2010年01期
【DOI】CNKI:SUN:SYJB.0.2010-01-017
【分類號】S567.3