【作者】郭佳; 宋榮; 包海鷹; 崔寶凱

【Author】GUO Jia1; SONG Rong1; BAO Haiying1; CUI Baokai21College of Traditional Medicine; Jilin Agricultural University; Changchun; Jilin 130118; China; 2Institute of Microbiology; Beijing Forestry University; Beijing 100083; China

【作者機構】吉林農業(yè)大學中藥材學院; 北京林業(yè)大學微生物研究所

【摘要】 選用H22荷瘤小鼠對火木層孔菌(Phellinus igniarius)和瓦尼木層孔菌(Phellinus vaninii)發(fā)酵產物進行抗腫瘤體內試驗,測定其對H22荷瘤小鼠抑瘤率、免疫器官指數(shù)、免疫因子含量和生存率的影響。結果表明,火木層孔菌和瓦尼木層孔菌發(fā)酵產物高、低劑量組(1 000 mg/kg、500 mg/kg)均對腫瘤具有一定抑制作用,其中瓦尼木層孔菌發(fā)酵產物低劑量組(500 mg/kg)抑瘤效果最佳,其抑瘤率為58.7%。火木層孔菌和瓦尼木層孔菌發(fā)酵產物高、低劑量組均可提高小鼠血清中白細胞介素2(interleukin-2,IL-2)的含量、延長小鼠的生存期,但是對血清中腫瘤壞死因子(tumor necrosis factor-,αTNF-α)含量的影響作用不顯著。

【Abstract】 Whole cultures(mycelium and spent broth) of Phellinus igniarius and Phellinus vaninii grown for 8 days at 30 ℃ on a medium containing sucrose,peptone and mineral salts,were evaporated to dryness in an oven for 24 h at 35 ℃.After grinding to a powder,the solid residues were suspended in physiological saline and the anti-tumor activity of the two preparations(FPPI-P.igniarius,and FPPV-P.vaninii) was determined using H22 tumor-bearing mice as the test model.Both low(500 mg/kg/d) and high(1 000 mg/kg/d) doses of FPPI and FPPV inhibited tumor growth.The inhibitory effect was most pronounced(58.7%) with low doses of FPPV,which was virtually identical to that recorded in the 5-fluorouracil(5-Fu) positive control group.Both low and high doses of FPPI and FPPV also increased interleukin-2(IL-2) levels in test mice and prolonged their survival time.

【關鍵詞】 火木層孔菌; 瓦尼木層孔菌; 抗腫瘤

【Key words】 Phellinus igniarius; Phellinus vaninii; antitumor activity

【基金】國家自然科學基金項目“藥用木腐真菌‘桑黃’資源及其抗腫瘤活性的研究”(編號:30870013)的部分研究內容
 

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2010年02期

【DOI】CNKI:SUN:SYJB.0.2010-02-017
 
【分類號】S567.39