【作者】 杜秀菊； 張勁松； 賈薇； 唐慶九； 劉艷芳； 杜秀云；
【Author】 DU Xiuju1,2,ZHANG Jingsong2 ,JIA Wei2,TANG Qingjiu2,LIU Yanfang2,DU Xiuyun31College of Life Science,Liaocheng University,Liaocheng,Shandong 252059,China;2National Engineering Research Center of Edible Fungi;Key Laboratory of Applied Mycological Resources and Utilization,Ministry of Agriculture;Shanghai Key Laboratory of Agricultural Genetics and Breeding;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;3Fenghuang Office,Dongchangfu District,Liaocheng,Shandong 252000,China
【機(jī)構(gòu)】 聊城大學(xué)生命科學(xué)學(xué)院； 國(guó)家食用菌工程技術(shù)研究中心農(nóng)業(yè)部應(yīng)用真菌資源與利用重點(diǎn)開(kāi)放實(shí)驗(yàn)室上海市農(nóng)業(yè)遺傳育種重點(diǎn)開(kāi)放實(shí)驗(yàn)室上海市農(nóng)業(yè)科學(xué)院食用菌研究所； 東昌府區(qū)鳳凰辦事處；
【摘要】 為篩選蛹蟲(chóng)草(Cordyceps militaris)子實(shí)體的抗腫瘤和免疫活性部位,采用系統(tǒng)溶劑法,分別用氯仿、乙酸乙酯、95%的無(wú)水乙醇、沸水(100℃)、3%草酸銨和5%NaOH依次提取并計(jì)算提取物得率,且測(cè)定了沸水提取物、草酸銨提取物、NaOH提取物1和2的多糖含量。采用體外抗腫瘤模型測(cè)定了氯仿提取物、乙酸乙酯提取物和乙醇提取物對(duì)腫瘤細(xì)胞L1210和SW620的抑制活性;采用體外免疫增殖試驗(yàn)?zāi)Ｐ蜏y(cè)定了沸水提取物、草酸銨提取物和NaOH提取物1和2的免疫活性。結(jié)果表明,乙酸乙酯提取物對(duì)腫瘤細(xì)胞L1210和SW620的抑制效果最好,當(dāng)乙酸乙酯提取物濃度為200μg/mL時(shí),抑制率分別為(85.2±2.5)%和(67.7±6.3)%;體外免疫活性測(cè)定結(jié)果表明,草酸銨提取物刺激小鼠脾淋巴細(xì)胞后的增殖活性最高,當(dāng)濃度為500μg/mL時(shí)細(xì)胞增殖率為(312.0±7.2)%,與陽(yáng)性對(duì)照PHA的細(xì)胞增殖率(329.3±3.0)%相當(dāng)。
【Abstract】 A systematic extraction system consisting of successive extractions with three organic solvents(chloroform,ethyl acetate,ethanol) followed by successive extractions with three non-organic solvents(distilled water at 100 ℃,3% ammonium oxalate solution,5% NaOH solution) was used to obtain bioactive components from dry,powdered C.militaris fruit bodies.Evaluation of the inhibitory effects of the organic extracts on L1210 and SW620 cancer cell lines revealed that ethyl acetate extracts were most eff...
【關(guān)鍵詞】 蛹蟲(chóng)草； 抗腫瘤； 免疫活性；
【Key words】 Cordyceps militaris； antitumor activity； immunostimulatory activity；
【基金】 國(guó)家自然科學(xué)基金(編號(hào):2006BAD06B08)的部分研究?jī)?nèi)容
【分類號(hào)】S567.35