凱氏定氮法測定靈芝破壁孢子粉中粗蛋白質含量的不確定度評定
Evaluation of the Uncertainty Associated with the Measurement of Protein in Ganoderma lucidum Wall-broken Spores Using the Kjeldahl Method

【作者】 王紅梅； 雷萍； 王少鷗； 劉海燕；

【Author】 WANG Hongmei,LEI Ping,WANG Shaoou,LIU Haiyan Institute for Agro-Food Standards and Testing Technology,Shanghai Academy of Agricultural Sciences, Supervision and Testing Center for Edible Fungi Quality at Shanghai,Ministry of Agriculture, Shanghai 201403,China

【機構】 上海市農業科學院農產品質量標準與檢測技術研究所,農業部食用菌產品質量監督檢驗測試中心(上海)；

【摘要】 對采用凱氏定氮法測定靈芝(Ganoderma lucidum)破壁孢子粉中粗蛋白含量的不確定度進行評定,結果表明合成相對標準不確定度為0.02%,擴展不確定度為0.04%。在本次孢子粉粗蛋白質測定中,引入不確定度的主要因素有鹽酸標準溶液的濃度及樣品測定中實際消耗的標準溶液體積。

【Abstract】 We evaluated the level of uncertainty associated with the measurement of crude protein in Ganoderma lucidum wall-broken spores using the Kjeldahl method.Our data revealed that the combined related standard uncertainty and the expanded uncertainty values were 0.02% and 0.04%,respectively.The level of uncertainty associated with the measurement of crude protein in G.lucidum wall-broken spores is mainly determined by the concentration of the standard hydrochloric acid solution and the volume of aci...

【關鍵詞】 凱氏定氮法； 靈芝破壁孢子粉； 粗蛋白質含量； 不確定度評定；
【Key words】 Kjeldahl method； Ganoderma lucidum wall-broken spores； crude protein content； uncertainty evaluation；

【分類號】TS219