董昌金; 王衛(wèi)東; 李濤
湖北師范學(xué)院生物系

【中文摘要】 該文通過(guò)對(duì)原生質(zhì)體形成與再生條件如酶類(lèi)、滲透壓穩(wěn)定劑、ｐＨ、培養(yǎng)基、菌齡、酶解溫度和時(shí)間等因素的研究表明，以Ｄ培養(yǎng)基、葡萄糖為滲透壓穩(wěn)定劑，酶液濃度為１保擔(dān)ィ蹋ｗ＋０保玻ィ蹋ｓ，ｐＨ４保怠５保擔(dān)培養(yǎng)菌齡為７２～９６ｈ，酶解溫度為２０～２５℃條件下，酶解６０～９０ｍｉｎ，其破壁效果為最佳，原生質(zhì)體形成和再生率都最高。

【英文摘要】 In the paper,the study of form and regeneration of protoplast for stabilizer,pH,culture medium,bacterial age,the temperature and time of enzyme effect show that the effective of broken cell wall is the best in the condition of using D culture medium glucose as stabilizer The temperature of enzyme effect is 20～25℃ The time of enzyme effect is 60～90min.The rate of form and regeneration of protoplast is the highest.

【中文關(guān)鍵詞】 原生質(zhì)體; 形成; 再生; 條件
【英文關(guān)鍵詞】 Protoplast; Form; Regeneration; Condition

【文獻(xiàn)出處】 中國(guó)食用菌,EDIBLE FUNGI OF CHINA,編輯部郵箱,1999年01期 【DOI】CNKI:SUN:ZSYJ.0.1999-01-002