雷萍; 黃志英; 林淼; 楊海華; 陳玉婷
上海市農(nóng)業(yè)科學(xué)院農(nóng)產(chǎn)品質(zhì)量標(biāo)準(zhǔn)與檢測技術(shù)研究所; 上海市飼料行業(yè)協(xié)會(huì); 上海儀表電子工業(yè)環(huán)境監(jiān)測站

【中文摘要】 本實(shí)驗(yàn)采用超高壓高效液相檢測法(UPLC)分離測定了多種蟲草產(chǎn)品中腺苷和蟲草素的含量。在最優(yōu)化條件下,以ACQUITY UPLC C18為色譜柱,流動(dòng)相為乙腈和水,等度洗脫,上述各組分在4 min內(nèi)可基本實(shí)現(xiàn)基線分離。各組分濃度與峰面積在117.5μg/mL范圍內(nèi)呈良好線性,檢測下限(S/N=3)分別為0.10μg/mL和0.12μg/mL。采用該方法成功測定了多種蟲草產(chǎn)品中腺苷和蟲草素的含量。

【英文摘要】 Adenosine and cordycepin levels in twelve Cordyceps products were successfully determined by Ultrahigh-Performance Liquid Chromatography(UPLC) using an ACQUITY C18 column,with water and acetonitrile as the mobile phase.Separation of the two components was achieved within four minutes,and the concentrations of each component showed a linear relationship with the peak area in the range 2～20 μg/mL.Detection limits(S/N=3) were 0.10 μg/mL and 0.12 μg/mL for adenosine and cordycepin,respectively.

【中文關(guān)鍵詞】 UPLC; 腺苷; 蟲草素
【英文關(guān)鍵詞】 Ultrahigh-Performance Liquid Chromatography; adenosine; cordycepin

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2009年03期 【DOI】CNKI:SUN:SYJB.0.2009-03-016